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The reaction was followed by acquiring 1D NMR experiments at 15-min intervals over 24 h. Gene functions were inferred from BLAST searches followed by gene linkage and cluster analysis. Deletion of yjhC resulted in loss of growth on 2,7-anhydro-Neu5Ac but not on Neu5Ac (Fig. 5C), which could be complemented in trans with yjhC (Fig If you have any type of ques...
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This was followed by the addition of neuraminidase solution or buffer control to each well and continued resistance measurement 4 (or more) h. AAV6 relies more on sialic acid or sialic acid-containing glycoproteins than AAV1 for cell entry and/or subsequent steps of infection. Neuraminidases are enzymes that cleave via hydrolysis α(2-3)-, α(2-6)-, and α(2-8)...