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Picture these moments: "Catering company, you'll need to plan for big appetites because this is a group of athletes." "DJ, let's put together a music program that appeals to the broad range of ages of guests." "Tent people, give us at least three ideas on how we can work around this sloped site." Leadership talent/impactful persona - This is one of those squ...
1 meseAttività comunitarie20050 - Via Nazario Sauro 2918 visualizzazioni
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Picture these moments: "Catering company, you'll need to plan for big appetites because this is a group of athletes." "DJ, let's put together a music program that appeals to the broad range of ages of guests." "Tent people, give us at least three ideas on how we can work around this sloped site." Leadership talent/impactful persona - This is one of those squ...
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1 meseAttività comunitarie39030 - Via Di Santa Melania 12614 visualizzazioni
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1 meseAttività comunitarie61832 - 355 Isaacs Creek Road17 visualizzazioni
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Mucin competition was carried out by pretreating rAAV with bovine submandibular gland mucin (Sigma) for 30 min at 20°C. AAV vectors, in the presence or absence of mucin, were added to Cos-7 cells in equal volumes of DMEM for 1 h at 37°C. Cells were rinsed twice with DMEM and incubated at 37°C. Two thousand particles/cell were pretreated with mucin (concentra...
1 meseAttività comunitarie39040 - Via San Domenico 7917 visualizzazioni
To confirm if both α2,3 and α2,6 sialic acids facilitate AAV1 and AAV6 transduction, we carried out a lectin competition assay on these three cell lines (Fig. 5A to C). In competitive binding assays, 10 mm free synthetic sialic acid (Sigma) was added 30 min before the endocytic agent and left during the assay. The supernatants were removed and used for total...
1 meseAttività comunitarie40048 - Via Vicenza 8515 visualizzazioni
CeceliaCho
1 mese
