Mucin competition was carried out by pretreating rAAV with bovine submandibular gland mucin (Sigma) for 30 min at 20°C. AAV vectors, in the presence or absence of mucin, were added to Cos-7 cells in equal volumes of DMEM for 1 h at 37°C. Cells were rinsed twice with DMEM and incubated at 37°C. Two thousand particles/cell were pretreated with mucin (concentrations up to 0. If you treasured this article therefore you would like to collect more info concerning manufacturer of sialic acid powder for Supplement Ingredients generously visit the webpage. 5 mg/ml), and cells were assayed for luciferase expression 24 h posttransduction.
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