Mucin competition was carried out by pretreating rAAV with bovine submandibular gland mucin (Sigma) for 30 min at 20°C. AAV vectors, in the presence or absence of mucin, were added to Cos-7 cells in equal volumes of DMEM for 1 h at 37°C. Cells were rinsed twice with DMEM and incubated at 37°C. To read more info in regards to manufacturer of sialic acid powder for pharmaceutical Ingredients visit the web site. Two thousand particles/cell were pretreated with mucin (concentrations up to 0.
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