US20110165626A1 - High Yield Production of Sialic Acid (Neu5ac) by Fermentation - Google Patents

US20110165626A1 - High Yield Production of Sialic Acid (Neu5ac) by Fermentation - Google Patents

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We stained cell lines with lectins that have been used to recognize the following three different epitopes: (i) WGA recognizes all sialic acids, (ii) MAA recognizes α2,3 sialic acid, and (iii) SNA recognizes α2,6 sialic acid. If you have almost any inquiries with regards to in which and how you can utilize manufacturer of sialic acid powder for drink Ingredients, it is possible to e-mail us with our own web site. In this report, we demonstrate that AAV1 and AAV6 use both α2,3 and α2,6 N-linked sialic acids for binding and infection. To analyze if the sialylated receptors of AAV1 and AAV6 are glycoproteins or glycolipids, we treated Cos-7 and Pro-5 cells with 200 μg/ml proteinase K. Cell viability was assessed immediately after proteinase K treatment, and no cytotoxic effect was observed. Leer más

Publicado el 2024/10/03

Descripción

We stained cell lines with lectins that have been used to recognize the following three different epitopes: (i) WGA recognizes all sialic acids, (ii) MAA recognizes α2,3 sialic acid, and (iii) SNA recognizes α2,6 sialic acid. If you have almost any inquiries with regards to in which and how you can utilize manufacturer of sialic acid powder for drink Ingredients, it is possible to e-mail us with our own web site. In this report, we demonstrate that AAV1 and AAV6 use both α2,3 and α2,6 N-linked sialic acids for binding and infection. To analyze if the sialylated receptors of AAV1 and AAV6 are glycoproteins or glycolipids, we treated Cos-7 and Pro-5 cells with 200 μg/ml proteinase K. Cell viability was assessed immediately after proteinase K treatment, and no cytotoxic effect was observed.

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