Background was washed out with deionized water and gel was scanned with the default instrument settings for analysis (Lexmark CX727 scanner). Inhibition of glycolipid synthesis was carried out as described previously (36) with some modifications. Resialylation was carried out with 50 mU/ml sialyltransferase in α-MEM for 2 h at 37°C. If you have any issues pertaining to where by and how to use Supplier of sialic acid powder as Raw Material for food, you can speak to us at our webpage. In the control groups, Lec-2 cells were untreated or incubated with either sialyltransferase or CMP-sialic acid. N-acetyl-neuraminic acid (2-keto-5-acetamido-3,5-dideoxy-D-glycero-D-galactononulopyranos-1-onic acid (often abbreviated as Neu5Ac, Neu5Ac, or NANA). Those of skill are aware that insertion of a nucleic acid into a chromosome can occur, e.g., by homologous recombination.
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