To assay for oxidoreductase activity, the purified recombinant proteins were incubated in 100-μl reactions at 37 °C overnight with 1 mg/ml 2,7-anhydro-Neu5Ac or Neu5Ac in 20 mm sodium phosphate buffer, pH 7. Should you adored this informative article in addition to you would like to be given more information regarding sialic acid powder supplier i implore you to visit the website. 5, in the presence 500 μm NADH. The structure was acquired from a crystal grown in the JCSG Plus screen (100 mm sodium citrate, pH 5.5, 20% PEG 3000). The diffraction experiment was performed on the I04 beamline at Diamond Light Source Ltd.
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