US20110165626A1 - High Yield Production of Sialic Acid (Neu5ac) by Fermentation - Google Patents

US20110165626A1 - High Yield Production of Sialic Acid (Neu5ac) by Fermentation - Google Patents

11.00 £
Casting - Audizioni
1 mese
Italia
Apulia
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3028 - Corso Garibaldi 26
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ID: 16803
Pubblicato il 1 mese fa da JulianaKunk
11.00 £
Nella categoria Casting - Audizioni
Corso Garibaldi 26, 3028 Contrada Cancelli Zona Industriale N.1, Apulia, Italia
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We stained cell lines with lectins that have been used to recognize the following three different epitopes: (i) WGA recognizes all sialic acids, (ii) MAA recognizes α2,3 sialic acid, and (iii) SNA recognizes α2,6 sialic acid. If you enjoyed this article and you would such as to receive additional details relating to sialic acid manufacturers kindly check out our webpage. In this report, we demonstrate that AAV1 and AAV6 use both α2,3 and α2,6 N-linked sialic acids for binding and infection. To analyze if the sialylated receptors of AAV1 and AAV6 are glycoproteins or glycolipids, we treated Cos-7 and Pro-5 cells with 200 μg/ml proteinase K. Cell viability was assessed immediately after proteinase K treatment, and no cytotoxic effect was observed. Leggi di più

Pubblicato il 2024/10/03

Descrizione

We stained cell lines with lectins that have been used to recognize the following three different epitopes: (i) WGA recognizes all sialic acids, (ii) MAA recognizes α2,3 sialic acid, and (iii) SNA recognizes α2,6 sialic acid. If you enjoyed this article and you would such as to receive additional details relating to sialic acid manufacturers kindly check out our webpage. In this report, we demonstrate that AAV1 and AAV6 use both α2,3 and α2,6 N-linked sialic acids for binding and infection. To analyze if the sialylated receptors of AAV1 and AAV6 are glycoproteins or glycolipids, we treated Cos-7 and Pro-5 cells with 200 μg/ml proteinase K. Cell viability was assessed immediately after proteinase K treatment, and no cytotoxic effect was observed.

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