Terminal Sialic Acids are an Important Determinant of Pulmonary Endothelial Barrier Integrity

To assay for oxidoreductase activity, the purified recombinant proteins were incubated in 100-μl reactions at 37 °C overnight with 1 mg/ml 2,7-anhydro-Neu5Ac or Neu5Ac in 20 mm sodium phosphate buffer, pH 7.5, in the presence 500 μm NADH. The structure was acquired from a crystal grown in the JCSG Plus screen (100 mm sodium citrate, pH 5.5, 20% PEG 3000). If you have any concerns concerning in which and how to use sialic acid powder suppliers, you can speak to us at our own site. The diffraction experiment was performed on the I04 beamline at Diamond Light Source Ltd. Crystal structure of RgNanOx. Read more