US20110165626A1 - High Yield Production of Sialic Acid (Neu5ac) by Fermentation - Google Patents

US20110165626A1 - High Yield Production of Sialic Acid (Neu5ac) by Fermentation - Google Patents

249.00 $
Обучение языкам
1 month
Italy
Basilicate
2060 - Rue Du Pont Simon 189
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ID: 16666
Published 1 month ago by IrisNewquis
249.00 $
Rue Du Pont Simon 189, 2060 Arlian, Basilicate, Italy
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In competitive binding assays, 10 mm free synthetic sialic acid (Sigma) was added 30 min before the endocytic agent and left during the assay. These were further purified by size-exclusion chromatography with elution in 50 mm KPi, 200 mm NaCl, pH 7.8, dialyzed against assay buffer (20 mm Tris, 150 mm NaCl, 2 mm tris(2-carboxyethyl)phosphine, pH 7.5), and finally concentrated to 0.5-1 mm for storage at 4 °C. All 1D NMR experiments were performed using a Bruker Advance I 500-MHz spectrometer with a 5-mm PATXI 1H/D-13C/15N Z-GRD probe at 293 K Should you beloved this informative article as well as you would want to be given more information about Supplier of sialic acid powder as Raw Material for drinks i implore you to check out our webpage. . Read more

Published on 2024/10/03

Description

In competitive binding assays, 10 mm free synthetic sialic acid (Sigma) was added 30 min before the endocytic agent and left during the assay. These were further purified by size-exclusion chromatography with elution in 50 mm KPi, 200 mm NaCl, pH 7.8, dialyzed against assay buffer (20 mm Tris, 150 mm NaCl, 2 mm tris(2-carboxyethyl)phosphine, pH 7.5), and finally concentrated to 0.5-1 mm for storage at 4 °C. All 1D NMR experiments were performed using a Bruker Advance I 500-MHz spectrometer with a 5-mm PATXI 1H/D-13C/15N Z-GRD probe at 293 K Should you beloved this informative article as well as you would want to be given more information about Supplier of sialic acid powder as Raw Material for drinks i implore you to check out our webpage. .

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IrisNewquis
Via Del Piave 124, 15022, Carentino, Case Olivieri, Aosta Valley, Italy
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