Shoyu, historically made from wheat and soy protein, has been produced in Japan for over 1,500 years, following its introduction from mainland China. For recovery of extracellular protein, reliable amino acids manufacturer the first separation of product from producing organisms is completed by centrifugation or membrane filtration. It is because of this that manufacturing instructions be specific, with most filtration pressures given. Process Types 1. Extraction and Isolation 1. Filtration -Ultrafiltration is commonly used to take away the desired product from the cell debris.