The reaction was followed by acquiring 1D NMR experiments at 15-min intervals over 24 h. All 1D NMR experiments were performed using a Bruker Advance I 500-MHz spectrometer with a 5-mm PATXI 1H/D-13C/15N Z-GRD probe at 293 K. To follow the kinetics of the reaction and assess the position of deuteration, two samples containing 2 mm 2,7-anhydro-Neu5Ac, 100 μm NADH, and 60 μm RgNanOx were used, one in deuterated PBS buffer (PBS/D2O) and one in standard PBS buffer (PBS/H2O, containing 10% D2O for locking purposes). Fractions were pooled and concentrated using a 10,000 molecular weight cut-off Vivaspin column (Vivaspin, Germany) If you adored this article therefore you would like to get more info about manufacturer of sialic acid powder as Raw Material for pharmaceuticals i implore you to visit our own page. .
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