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In competitive binding assays, 10 mm free synthetic sialic acid (Sigma) was added 30 min before the endocytic agent and left during the assay. These were further purified by size-exclusion chromatography with elution in 50 mm KPi, 200 mm NaCl, pH 7.8, dialyzed against assay buffer (20 mm Tris, 150 mm NaCl, 2 mm tris(2-carboxyethyl)phosphine, pH 7.5), and fin...
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3 mesiCase - Appartamenti in Affitto83400 - 19 Rue Des Lacs34 visualizzazioni
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3 mesiPubblicità - Relazioni Pubbliche54027 - Via Santa Maria Di Costantinopoli 7520 visualizzazioni
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3 mesi
