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Picture these moments: "Catering company, you'll need to plan for big appetites because this is a group of athletes." "DJ, let's put together a music program that appeals to the broad range of ages of guests." "Tent people, give us at least three ideas on how we can work around this sloped site." Leadership talent/impactful persona - This is one of those squ...
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Picture these moments: "Catering company, you'll need to plan for big appetites because this is a group of athletes." "DJ, let's put together a music program that appeals to the broad range of ages of guests." "Tent people, give us at least three ideas on how we can work around this sloped site." Leadership talent/impactful persona - This is one of those squ...
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Mucin competition was carried out by pretreating rAAV with bovine submandibular gland mucin (Sigma) for 30 min at 20°C. AAV vectors, in the presence or absence of mucin, were added to Cos-7 cells in equal volumes of DMEM for 1 h at 37°C. Cells were rinsed twice with DMEM and incubated at 37°C. Two thousand particles/cell were pretreated with mucin (concentra...
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To confirm if both α2,3 and α2,6 sialic acids facilitate AAV1 and AAV6 transduction, we carried out a lectin competition assay on these three cell lines (Fig. 5A to C). In competitive binding assays, 10 mm free synthetic sialic acid (Sigma) was added 30 min before the endocytic agent and left during the assay. The supernatants were removed and used for total...
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