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Neu5Ac (blue), 2,7-anhydro-Neu5Ac (orange), or XY intermediate (gray). B and C, superposition of the 1H,13C HSQC reference spectra of Neu5Ac (blue) and 2,7-anhydro-Neu5Ac (orange) and 1H,13C HSQC reaction mixture at 30 min (where the XY peak is observed to peak, gray) If you loved this short article and you would like to acquire extra facts with regards to m...
The reaction was followed by acquiring 1D NMR experiments at 15-min intervals over 24 h. Gene functions were inferred from BLAST searches followed by gene linkage and cluster analysis. Deletion of yjhC resulted in loss of growth on 2,7-anhydro-Neu5Ac but not on Neu5Ac (Fig. 5C), which could be complemented in trans with yjhC (Fig. 5D), suggesting that the ge...
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