Background was washed out with deionized water and gel was scanned with the default instrument settings for analysis (Lexmark CX727 scanner). Inhibition of glycolipid synthesis was carried out as described previously (36) with some modifications. Resialylation was carried out with 50 mU/ml sialyltransferase in α-MEM for 2 h at 37°C. If you have any issues wi...
Different AAV serotypes display distinct tissue tropism, believed to be related to the distribution of their receptors on target cells. The AAV serotype helper plasmids pXR1, pXR2, pXR4, and pXR5, as well as the plasmid pXX6-80 containing the adenovirus helper genes for AAV replication, have been described elsewhere (32, 47). AAV6 helper plasmid pXR6 was kin...
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