Similar to a Previous Study (19)

Similar to a Previous Study (19)

187.00 £
Antiquitäten
1 month
Italia
Campania
Insediamento di Via Ghiardo 3/9 Get directions →
Ex15 7ld - 27 Seaford Road
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ID: 16726
Published 1 month ago by IrisNewquis
187.00 £
In Antiquitäten category
27 Seaford Road, Ex15 7ld Insediamento di Via Ghiardo 3/9, Campania, Italia
Get directions →
9 item views
Whole cell lysate (20 μl) was combined with 80 μl of 0.05 N H2SO4 (hydrolysis reagent) and incubated at 80°C for 60 min. Samples were briefly centrifuged at 14,000 revolution/min (16,000 g), after which 20 μl of 1 M NaOH (neutralization reagent) was added and the mixture centrifuged again at 14,000 revolution/min. Afterwards, wells were washed 3 times, 100 µl of substrate solution was added for 30 min at RT and the optical density (OD) was measured at 405 nm by an ELISA reader (TECAN Infinite 200 PRO). If you cherished this article so you would like to receive more info relating to Supplier of sialic acid powder for drink Ingredients generously visit our webpage. The wells were washed 3 times and then 100 µl of a specific alkaline phosphatase labelled antibody for terminal complement complex (TCC, C5b-9; provided by the manufacturer) was added and incubated for 30 min at RT. Read more

Published on 2024/10/03

Description

Whole cell lysate (20 μl) was combined with 80 μl of 0.05 N H2SO4 (hydrolysis reagent) and incubated at 80°C for 60 min. Samples were briefly centrifuged at 14,000 revolution/min (16,000 g), after which 20 μl of 1 M NaOH (neutralization reagent) was added and the mixture centrifuged again at 14,000 revolution/min. Afterwards, wells were washed 3 times, 100 µl of substrate solution was added for 30 min at RT and the optical density (OD) was measured at 405 nm by an ELISA reader (TECAN Infinite 200 PRO). If you cherished this article so you would like to receive more info relating to Supplier of sialic acid powder for drink Ingredients generously visit our webpage. The wells were washed 3 times and then 100 µl of a specific alkaline phosphatase labelled antibody for terminal complement complex (TCC, C5b-9; provided by the manufacturer) was added and incubated for 30 min at RT.

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IrisNewquis
Via Del Piave 124, 15022, Carentino, Case Olivieri, Aosta Valley, Italia
137 active listings
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Last online 1 month ago
Registered for 1 month

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