Whole cell lysate (20 μl) was combined with 80 μl of 0.05 N H2SO4 (hydrolysis reagent) and incubated at 80°C for 60 min. Samples were briefly centrifuged at 14,000 revolution/min (16,000 g), after which 20 μl of 1 M NaOH (neutralization reagent) was added and the mixture centrifuged again at 14,000 revolution/min. If you have any questions concerning where b...
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