However the nation was turning into extra affluent and consumers had been willing to pay a bit of more for their favorite seasoning, so between 1964 and 1970 the foremost makers (Kikkoman, Yamasa, Higeta) all stopped utilizing HVP and custom amino acids manufacturer returned to making fermented shoyu, although typically 85% of the soybeans have been defatted. For 5-10 years after the struggle, little fermented shoyu was made in Japan; its manufacturing reached a low in 1949, with most being semichemical or chemical. Endogenous retroviruses may be present in hybridomas used for monoclonal antibody manufacturing. It is fascinating to notice that right now and up to the current in America, the most widely used soy sauce was nonfermented HVP soy sauce, the poorest grade in Japan, which can be discontinued altogether there in the 1970s. By 1964 solely 0.2% of all Japanese shoyu was exported, however the quantity was growing.