Shoyu, historically made from wheat and soy protein, has been produced in Japan for over 1,500 years, following its introduction from mainland China. For restoration of extracellular protein, the primary separation of product from producing organisms is completed by centrifugation or membrane filtration. It is because of this that manufacturing instructions be specific, with maximum filtration pressures given. Process Types 1. Extraction and amino acids supplier Europe Isolation 1. Filtration -Ultrafiltration is often used to remove the desired product from the cell debris.