Neuraminidases and other reagents were obtained from Sigma-Aldrich. If you cherished this article and you would like to obtain extra facts pertaining to manufacturer of sialic acid powder as Raw Material for drinks kindly pay a visit to the webpage. To determine whether sialic acids play a role in endothelial barrier function, cells were treated with neuraminidases to hydrolyze sialic acid moieties. Two components of glycocalyx structure that may play a role in determining function in glycoproteins are 1) the terminal sugar residue(s) on the oligosaccharide chain(s) and 2) the amino acid residue attachment site (N- vs.