Shoyu, historically made from wheat and soy protein, has been produced in Japan for over 1,500 years, following its introduction from mainland China. amino acids manufacturer for supplements recovery of extracellular protein, the first separation of product from producing organisms is accomplished by centrifugation or membrane filtration. It is for that reason that manufacturing instructions be specific, with most filtration pressures given. Process Types 1. Extraction and Isolation 1. Filtration -Ultrafiltration is usually used to take away the specified product from the cell debris.