The reaction was followed by acquiring 1D NMR experiments at 15-min intervals over 24 h. All 1D NMR experiments were performed using a Bruker Advance I 500-MHz spectrometer with a 5-mm PATXI 1H/D-13C/15N Z-GRD probe at 293 K. To follow the kinetics of the reaction and assess the position of deuteration, two samples containing 2 mm 2,7-anhydro-Neu5Ac, 100 μm NADH, and 60 μm RgNanOx were used, one in deuterated PBS buffer (PBS/D2O) and one in standard PBS buffer (PBS/H2O, containing 10% D2O for locking purposes). Fractions were pooled and concentrated using a 10,000 molecular weight cut-off Vivaspin column (Vivaspin, Germany) If you beloved this report and you would like to acquire more information pertaining to manufacturer of sialic acid powder as Raw Material for cosmetics kindly stop by the web site. .
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