The worth of creating intensive, tissue-specific maps of protein lysine acetylation sites is further underscored by the numerous role of posttranslational modifications within the evolution and phenotypic composition of vertebrates, as indicated by the significant selective stress gene regulatory components close to posttranslational-modifying enzymes have come beneath in placental animals ( Lowe et al., 2011 ). Here, legal practice we combined an efficient method for lawyer protein extraction from tissue samples with lysine-acetylated peptide immunoprecipitation and excessive-accuracy tandem mass spectrometric (MS/MS) measurements, lawyer which allowed us to generate an atlas of lysine acetylation sites in 16 completely different rat tissues.
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