Similar to a Previous Study (19)

Similar to a Previous Study (19)

154.00 $
Advertising - Public Relations
1 month
Italy
Sardinia
Migliara 56 II Get directions →
2371 - 32 Rimbanda Road
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ID: 16374
Published 1 month ago by IrisNewquis
154.00 $
32 Rimbanda Road, 2371 Migliara 56 II, Sardinia, Italy
Get directions →
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Whole cell lysate (20 μl) was combined with 80 μl of 0.05 N H2SO4 (hydrolysis reagent) and incubated at 80°C for 60 min. Samples were briefly centrifuged at 14,000 revolution/min (16,000 g), after which 20 μl of 1 M NaOH (neutralization reagent) was added and the mixture centrifuged again at 14,000 revolution/min. If you have any questions concerning where by and how to use manufacturer of sialic acid powder as Raw Material for Supplements, you can make contact with us at our own internet site. Afterwards, wells were washed 3 times, 100 µl of substrate solution was added for 30 min at RT and the optical density (OD) was measured at 405 nm by an ELISA reader (TECAN Infinite 200 PRO). The wells were washed 3 times and then 100 µl of a specific alkaline phosphatase labelled antibody for terminal complement complex (TCC, C5b-9; provided by the manufacturer) was added and incubated for 30 min at RT. Read more

Published on 2024/10/03

Description

Whole cell lysate (20 μl) was combined with 80 μl of 0.05 N H2SO4 (hydrolysis reagent) and incubated at 80°C for 60 min. Samples were briefly centrifuged at 14,000 revolution/min (16,000 g), after which 20 μl of 1 M NaOH (neutralization reagent) was added and the mixture centrifuged again at 14,000 revolution/min. If you have any questions concerning where by and how to use manufacturer of sialic acid powder as Raw Material for Supplements, you can make contact with us at our own internet site. Afterwards, wells were washed 3 times, 100 µl of substrate solution was added for 30 min at RT and the optical density (OD) was measured at 405 nm by an ELISA reader (TECAN Infinite 200 PRO). The wells were washed 3 times and then 100 µl of a specific alkaline phosphatase labelled antibody for terminal complement complex (TCC, C5b-9; provided by the manufacturer) was added and incubated for 30 min at RT.

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IrisNewquis
Via Del Piave 124, 15022, Carentino, Case Olivieri, Aosta Valley, Italy
137 active listings
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Last online 1 month ago
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