Neuraminidases and other reagents were obtained from Sigma-Aldrich. If you liked this write-up and you would like to obtain additional details pertaining to china n-acetylneuraminic acid powder kindly visit our own web-site. To determine whether sialic acids play a role in endothelial barrier function, cells were treated with neuraminidases to hydrolyze sialic acid moieties. Two components of glycocalyx structure that may play a role in determining function in glycoproteins are 1) the terminal sugar residue(s) on the oligosaccharide chain(s) and 2) the amino acid residue attachment site (N- vs.