Shoyu, traditionally made from wheat and soy protein, has been produced in Japan for over 1,500 years, following its introduction from mainland China. amino acids manufacturer for pharmaceuticals recovery of extracellular protein, the primary separation of product from producing organisms is achieved by centrifugation or membrane filtration. It's for this reason that manufacturing instructions be specific, with most filtration pressures given. Process Types 1. Extraction and Isolation 1. Filtration -Ultrafiltration is often used to take away the specified product from the cell debris. The standard isolation of BDP should be of the same control because the atmosphere used for the formulation of the solution previous to sterilization and filling.