For experiments, PC-12 cells were collected via 0. If you enjoyed this information and you would like to receive more details regarding manufacturer of sialic acid powder for Supplement Ingredients kindly see our web-site. 25% trypsin (Gibco) and seeded for culturing in collagen IV (Sigma-Aldrich) coated 4-chamber slides (Nunc Lab-Tek, Merck). PC-12 cells grew as small clusters in suspension and were maintained in a density 5 × 105 cells/ml in culture flasks coated by 0.1% collagen type IV (Sigma-Aldrich). For testing bioactivity of polySia avDP20, murine embryonic stem cell-derived microglia (ESdM) cells were seeded in 1.
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