To assay for oxidoreductase activity, the purified recombinant proteins were incubated in 100-μl reactions at 37 °C overnight with 1 mg/ml 2,7-anhydro-Neu5Ac or Neu5Ac in 20 mm sodium phosphate buffer, pH 7.5, in the presence 500 μm NADH. The structure was acquired from a crystal grown in the JCSG Plus screen (100 mm sodium citrate, pH 5. If you have any concerns relating to in which along with how you can utilize Supplier of sialic acid powder as Raw Material for drinks, you possibly can e-mail us from the site. 5, 20% PEG 3000).