US20110165626A1 - High Yield Production of Sialic Acid (Neu5ac) by Fermentation - Google Patents

US20110165626A1 - High Yield Production of Sialic Acid (Neu5ac) by Fermentation - Google Patents

105.00 $
Car Parts
1 month
Italy
Campania
6830 - Rue De Fontigny 313
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ID: 16215
Published 1 month ago by CasimiraLor
105.00 $
In Car Parts category
Rue De Fontigny 313, 6830 Nusenna, Campania, Italy
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We stained cell lines with lectins that have been used to recognize the following three different epitopes: (i) WGA recognizes all sialic acids, (ii) MAA recognizes α2,3 sialic acid, and (iii) SNA recognizes α2,6 sialic acid. In this report, we demonstrate that AAV1 and AAV6 use both α2,3 and α2,6 N-linked sialic acids for binding and infection. To analyze if the sialylated receptors of AAV1 and AAV6 are glycoproteins or glycolipids, we treated Cos-7 and Pro-5 cells with 200 μg/ml proteinase K. If you loved this report and you would like to get a lot more facts relating to n-acetylneuraminic acid factory kindly check out our webpage. Cell viability was assessed immediately after proteinase K treatment, and no cytotoxic effect was observed. Read more

Published on 2024/10/03

Description

We stained cell lines with lectins that have been used to recognize the following three different epitopes: (i) WGA recognizes all sialic acids, (ii) MAA recognizes α2,3 sialic acid, and (iii) SNA recognizes α2,6 sialic acid. In this report, we demonstrate that AAV1 and AAV6 use both α2,3 and α2,6 N-linked sialic acids for binding and infection. To analyze if the sialylated receptors of AAV1 and AAV6 are glycoproteins or glycolipids, we treated Cos-7 and Pro-5 cells with 200 μg/ml proteinase K. If you loved this report and you would like to get a lot more facts relating to n-acetylneuraminic acid factory kindly check out our webpage. Cell viability was assessed immediately after proteinase K treatment, and no cytotoxic effect was observed.

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