The internalized bacteria were estimated by flow cytometry, by measuring the mean fluorescence intensity (MFI) of the cells. If you're ready to find more information on manufacturer of sialic acid powder for Supplement Ingredients review our web page. The phagocytosis was analysed by flow cytometry or confocal microscopy. Images were assembled and analysed with the Leica Confocal software LCS Lite 2.6 (Leica Microsystem). Images were acquired with a TCS SP2 AOBS confocal microscope (Leica Microsystem, Mannheim, GmbH, Deutschland) with × 40 oil immersion optics and 488 nm and 633 nm laser lines for FITC and Alexa Fluor 633 excitation, respectively.
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