Mucin competition was carried out by pretreating rAAV with bovine submandibular gland mucin (Sigma) for 30 min at 20°C. AAV vectors, in the presence or absence of mucin, were added to Cos-7 cells in equal volumes of DMEM for 1 h at 37°C. Cells were rinsed twice with DMEM and incubated at 37°C. Two thousand particles/cell were pretreated with mucin (concentra...
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To confirm if both α2,3 and α2,6 sialic acids facilitate AAV1 and AAV6 transduction, we carried out a lectin competition assay on these three cell lines (Fig. 5A to C). In competitive binding assays, 10 mm free synthetic sialic acid (Sigma) was added 30 min before the endocytic agent and left during the assay. The supernatants were removed and used for total...
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Coinbase is a digital asset exchange company founded in 2012. They offer trading of Bitcoin, Ethereum, Litecoin, and other digital assets with fiat currencies in 32 countries, and bitcoin transactions and storage in 190 countries worldwide. You can buy Catcoin directly through the Coinbase Wallet app on your mobile phone if you are using Coinbase Wallet. Swe...
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