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1 meseCorsi Lingue Straniere60-103 - Ul. Gostynska 14713 visualizzazioni
In competitive binding assays, 10 mm free synthetic sialic acid (Sigma) was added 30 min before the endocytic agent and left during the assay. These were further purified by size-exclusion chromatography with elution in 50 mm KPi, 200 mm NaCl, pH 7.8, dialyzed against assay buffer (20 mm Tris, 150 mm NaCl, 2 mm tris(2-carboxyethyl)phosphine, pH 7.5), and fin...
4 mesiCorsi Lingue Straniere2060 - Rue Du Pont Simon 18932 visualizzazioni
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