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HepG2 (A) and Pro-5 (B) cells were transduced with a constant amount of AAV1-luc or AAV6-luc vectors in the presence of a 200-fold-excess amount of competing AAV1, AAV6, or AAV2 encapsidated λ phage DNA-containing vector at 37°C for 1 h. However, more and more studies suggest that AAV2-based vectors are rate limiting in certain tissues (5, 32). If you enjoye...
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The first step of AAV infection often requires the attachment of the capsid to carbohydrate moieties on the cell surface. Of the 11 well-characterized AAV serotypes, heparan sulfate proteoglycan and sialic acid have been suggested to be the attachment receptors for AAV type 2 and types 4 and 5, respectively. In this report, we identify the receptor for the t...
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