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Treatment of isolated-perfused lungs with neuraminidase from Vibrio cholerae leads to pulmonary edema. A third key observation in our study is the pattern of alveolar edema observed when isolated-perfused lungs were treated with neuraminidase from Vibrio cholerae. A second key observation in our study is that terminally linked sialic acids are important cont...
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Neuraminidases and other reagents were obtained from Sigma-Aldrich. To determine whether sialic acids play a role in endothelial barrier function, cells were treated with neuraminidases to hydrolyze sialic acid moieties. Two components of glycocalyx structure that may play a role in determining function in glycoproteins are 1) the terminal sugar residue(s) o...
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