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To obtain tradition extract, a colony was transferred to 10 ml of TSB broth and incubated overnight at 37 °C. The micro organism have been then cultured in excessive volume of TSB and incubated at 37 °C. The bacteria have been proliferated in TSB broth medium and after overnight incubation, was streaked on the blood agar medium. •B. coagulans can scale back ...
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