Mucin competition was carried out by pretreating rAAV with bovine submandibular gland mucin (Sigma) for 30 min at 20°C. AAV vectors, in the presence or absence of mucin, were added to Cos-7 cells in equal volumes of DMEM for 1 h at 37°C. Cells were rinsed twice with DMEM and incubated at 37°C. Two thousand particles/cell were pretreated with mucin (concentra...
1 monthCommunity Veranstaltungen39040 - Via San Domenico 7918 views
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Exploring the Charm of the reallifecanm: A Quick look into Internet-based Peep Culture In the vast field of the internet, where information streams unceasingly and the limits of human collaboration are incessantly changing, a peculiar underground culture has arisen that beguilles the adventurous minds of netizens – the voteurhousetv. The reallifecams, common...
2 monthsCommunity Veranstaltungen61040 - Via Silvio Spaventa 5423 views
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