We stained cell lines with lectins that have been used to recognize the following three different epitopes: (i) WGA recognizes all sialic acids, (ii) MAA recognizes α2,3 sialic acid, and (iii) SNA recognizes α2,6 sialic acid. In this report, we demonstrate that AAV1 and AAV6 use both α2,3 and α2,6 N-linked sialic acids for binding and infection. To analyze i...
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This was followed by the addition of neuraminidase solution or buffer control to each well and continued resistance measurement 4 (or more) h. AAV6 relies more on sialic acid or sialic acid-containing glycoproteins than AAV1 for cell entry and/or subsequent steps of infection. Neuraminidases are enzymes that cleave via hydrolysis α(2-3)-, α(2-6)-, and α(2-8)...
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Biol. 48:443 (1970), by the search for similarity method of Pearson & Lipman, Proc. Cell. Biol. When you liked this short article along with you would want to acquire details regarding n-acetylneuraminic acid factory generously check out our web-site. 4:1440-1448) ADH2 (Russell et al. "Conservatively modified variations" of a particular polynucleotide se...
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