This observation was further supported by the staining with fluorescently tagged lectin from Arachis hypogaea following neuraminidase treatment. TRITC-tagged lectin from Arachis hypogaea did not bind to treated cells (B), and the monolayer was not disrupted (C). Treated cells exhibited severely disrupted monolayers. B: Confluent monolayers of PAECs and PMVEC...
In some experiments the bacterial surface was desialylated with 13· In the earlier set of experiments that utilized neuraminidase, we noted that, following neuraminidase treatment, there were typically fewer cells in the dish, indicating that cells had lost cell-cell and/or cell-matrix adhesions. If you have any questions regarding in which and how to use Su...
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