Shoyu, historically made from wheat and soy protein, has been produced in Japan for over 1,500 years, following its introduction from mainland homepage China. For recovery of extracellular protein, the first separation of product from producing organisms is completed by centrifugation or membrane filtration. It's for that reason that manufacturing instructions be specific, with maximum filtration pressures given. Process Types 1. Extraction and Isolation 1. Filtration -Ultrafiltration is usually used to take away the desired product from the cell debris.