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Background was washed out with deionized water and gel was scanned with the default instrument settings for analysis (Lexmark CX727 scanner). Inhibition of glycolipid synthesis was carried out as described previously (36) with some modifications. Resialylation was carried out with 50 mU/ml sialyltransferase in α-MEM for 2 h at 37°C. If you have any issues pe...
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