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Emilia-Romagna

This was followed by the addition of neuraminidase solution or buffer control to each well and continued resistance measurement 4 (or more) h. AAV6 relies more on sialic acid or sialic acid-containing glycoproteins than AAV1 for cell entry and/or subsequent steps of infection. Neuraminidases are enzymes that cleave via hydrolysis α(2-3)-, α(2-6)-, and α(2-8)...

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Global (United States, European Union and China) Sialic Acid Market Research Report 2024-2024

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To confirm if both α2,3 and α2,6 sialic acids facilitate AAV1 and AAV6 transduction, we carried out a lectin competition assay on these three cell lines (Fig. 5A to C). In competitive binding assays, 10 mm free synthetic sialic acid (Sigma) was added 30 min before the endocytic agent and left during the assay. The supernatants were removed and used for total...

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This observation was further supported by the staining with fluorescently tagged lectin from Arachis hypogaea following neuraminidase treatment. TRITC-tagged lectin from Arachis hypogaea did not bind to treated cells (B), and the monolayer was not disrupted (C). Treated cells exhibited severely disrupted monolayers. B: Confluent monolayers of PAECs and PMVEC...

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